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HPLC法测定地克珠利预混剂的含量
[摘要] 目的: 用HPLC法测定地克珠利预混剂的含量。方法: 采用Kromasil C18色谱柱(5u,150×4.6mm),乙腈-0.2%磷酸溶液(57:43)PH:2.51为流动相,检测波长为280nm。结果:地克珠利在0.1~0.5mg/mL浓度范围内线性关系良好,回归方程:Y=154260X+27797 r=0.997(n=5),平均回收率为:99.5% RSD:0.6%(n=5)。结论:HPLC法测定地克珠利预混剂的含量灵敏度高、方法简便、准确性好。
[关键词] HPLC(高效液相色谱法);地克珠利预混剂;含量测定

解答:

Determination of the content of diclazuril pre-coagulant by HPLC methodology

[ABSTRACT] Aim: determination the content of diclazuril pre-coagulant by HPLC methodology. Measurements: Application of Kromasil C18 chromatographic column (5u, 150×4.6mm) and acetonitrile-0.2% phosphoric acid solution (57:43) in pH=2.51 as mobile phase, and in measurements at 280nm as scanning wavelength. Result: diclazuril illustrates good linear correlation with the concentration in range of 0.1~0.5mg/mL, and the regression equation is Y=154260X+27797, r=0.997(n=5), with the average regression ratio of 99.5% under the RSD (Relative Standard Deviation) of 0.6% (n=5). Conclusion: Determination of the content of diclazuril pre-coagulant applied by HPLC methodology is a simple and convenient methodology with high sensitivity and accuracy.

[Key Words] HPLC(high performance liquid chromatography); diclazuril pre-coagulant; content determination/measurements